PLS-UV spectrophotometric method for the simultaneous determination of paracetamol, acetylsalicylic acid and caffeine in pharmaceutical formulations
Z. Bouhsain1, Salvador Garrigues1 and M. de la Guardia1
(1) Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, 50 Dr, Moliner St, E-46100, Burjassot (Valencia), Spain, ES
Abstract A simple and fast analytical procedure is proposed for the simultaneous determination of paracetamol, acetylsalicylic acid and caffeine in pharmaceuticals by means the partial least square treatment of the spectrophotometric absorbance data between 216 and 300 nm, taken at 5 nm intervals. The method involves the use of 8 standard mixtures of the three compounds assayed, considered at two concentration levels, and the measurement of the absorbance of samples in a 20% (v/v) ethanol in water solution previously filtered. In the analysis of real and synthetic samples precise and accurate values were obtained by the aforementioned procedure, providing in all cases variation coefficients and accuracy errors lower than 5% which agree with the tolerance level established by the pharmacopoeia for this kind of samples which is ±10%.
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